The Multicolor Fluorescence in Situ Hybridization (mFISH) Homepage
نویسندگان
چکیده
منابع مشابه
Multicolor 3D fluorescence in situ hybridization for imaging interphase chromosomes.
Fluorescence in situ hybridization (FISH) of specific DNA probes has become a widely used technique mostly for chromosome analysis and for studies of the chromosomal location of specific DNA segments in metaphase preparations as well as in interphase nuclei. FISH on 3D-preserved nuclei (3D-FISH) in combination with 3D-microscopy and image reconstruction is an efficient tool to analyze the spati...
متن کاملKaryotype refinement by multicolor fluorescence in situ hybridization analysis in 18 patients with acute lymphoblastic leukemia.
Eighteen patients with acute lymphoblastic leukemia (ALL) were investigated using spectral karyotyping (SKY), and multicolor fluorescence in situ hybridization (FISH) banding analyses. SKY analysis confirmed previous results in patients with G-banded diploid karyotype. Nevertheless in three out of five patients with two or more chromosomal abnormalities, SKY and multicolor banding methods refin...
متن کاملClassification of Multicolor Fluorescence in Situ Hybridization Images Using Gaussian Mixture Models
This paper introduces a fully automatic chromosome classification algorithm for Multicolor or Multiplex Fluorescence In-Situ Hybridization (M-FISH) images using Gaussian mixture model technique. M-FISH is a recently developed cellular imaging method for rapid detection of chromosomal abnormalities, where each chromosome is labeled with 5 dyes and counterstained with DAPI florescence stain. The ...
متن کاملNormalization of multicolor fluorescence in situ hybridization (M-FISH) images for improving color karyotyping.
BACKGROUND Multiplex or multicolor fluorescence in situ hybridization (M-FISH) is a recently developed cytogenetic technique for cancer diagnosis and research on genetic disorders. By simultaneously viewing the multiply labeled specimens in different color channels, M-FISH facilitates the detection of subtle chromosomal aberrations. The success of this technique largely depends on the accuracy ...
متن کاملTips and Tricks in Fluorescence In-situ Hybridization (FISH)- based Preimplantation Genetic Diagnosis /Screening (PGD/PGS)
As numerical and structural defects in chromosomes are an inevitable consequence of IVF, Pre-implantation genetic diagnosis and screening (PGD/PGS) methods are used for detecting abnormalities in embryos before implantation to the uterus to increase the successful rate of IVF. Pre-implantation genetic diagnosis and screening approaches can be achieved by different techniques such as NGS, CGH an...
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ژورنال
عنوان ژورنال: Balkan Journal of Medical Genetics
سال: 2008
ISSN: 1311-0160
DOI: 10.2478/v10034-008-0014-0